The three-times a week service will be served by Australian-flagged and crewed vessels, with the first being a 516 TEU multi-purpose self-geared container ship, the MV Tassie Bridge.
A second vessel might be added if there is sufficient demand.
Agility Shipping's vessels will operate on fixed berthing windows at Webb Dock 4 in Port of Melbourne and Bell Bay in Tasmania.
Stevedoring and container depot operations will be conducted by Patrick Stevedores in Melbourne and by ANS Stevedores in Bell Bay, both under long-term agreements.
Agility provides a full range of supply chain solutions to customers across Australia.
The joint venture agreement between the two parties was signed in Melbourne on July 23.