The port, on the Mississippi River in Northwest Tennessee, is near several key transportation routes.
It is some 30 miles away from rail lines connecting Canada to the Gulf of Mexico and about 25 miles from Interstate 155 highway.
It is also around 28 miles from the future route of I-69 highway, which will serve 17 of the nation's top 25 seaports when built.
"With this site's prime location, our area's workforce and the potential to recruit new industry, our region is poised to be a real economic force in our country, and Tennessee's working families will benefit," said Congressman John Tanner in a statement.
"The Tiger program is an important one to invest in the creation of private-sector job growth, and we are pleased that others realize the important role Northwest Tennessee plays in our country’s economic future."
According to the statement, funds for the Tiger program come under the American Recovery and Reinvestment Act of 2009, also known as the US "stimulus bill".
When constructed, the port will create up to 2,300 jobs, according to research from the Business and Economic Research Center at Middle Tennessee State University.
The award for the project will be officially announced by the US Department of Transportation on October 20.